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Investigation of Antiproliferative Effect of Phenethyl Isothiocyanate on High-Grade Hepatocellular Carcinoma in vitro
Ayşe Burçin UyumluDepartment of Biochemistry, Faculty of Pharmacy, Inonu University, Malatya, TürkiyeObjectives: Hepatocellular carcinoma (HCC) is a primary liver tumor that exhibits significant resistance to conventional chemotherapeutic treatments. Phenethyl isothiocyanate (PEITC), a phytochemical derived from Cruciferae plants, has emerged as a promising therapeutic candidate. We aimed to assess the effects of PEITC on cell proliferation and migration in the SNU-449 HCC cells.
Methods: To evaluate the effect of PEITC (2.5-100 µM) on cell viability and migratory rate in SNU-449 cells, MTT, Sulforhodamine B (SRB), colony formation and wound healing assays were analyzed. All data are presented as the median and interquartile range (IQR).
Results: The results indicated that cell viability in response to PEITC began to decrease at 10 µM after 72 hours, with absorbance values of 0.431 (IQR: 0.458) for the 10 µM treatment and 0.67 (IQR: 0.049) for the control group, respectively (p<0.05). The SRB assay results for PEITC-treated and control groups were 0.581 (IQR: 0.789) and 0.381 (IQR: 0.365), respectively (p>0.05). The surviving fraction of cells treated with PEITC was 19.35% relative to untreated controls. Wound closure percentages in the PEITC and control groups were 10.35% (IQR: 10.3) and 59.75% (IQR: 15.4), respectively (p<0.05).
Conclusion: Our results suggest that PEITC may be beneficial in ameliorating hepatocellular carcinoma. Further comprehensive studies are planned to elucidate the molecular mechanisms underlying the antitumoral effect of PEITC.
Manuscript Language: English